Eubank-Watson II. One uppercut ends two careers - Watson for being put in a wheelchair, and Eubank for never wanting to repeat that damage for the rest of his career.
Great responses and a few I would have missed. On the Weaver-Tate fight, I think the game-changing punch was actually the body shot right before the left hook. Weaver pushes Big John into the ropes and when he bounces back there's a brutal body shot. You can see all the air go out of Tate, and then comes the finisher. I believe without the body shot first he survives the round. Tate goes into obscurity, probably robbing Larry Holmes of a big payday unification as well as a fight that would have added to his fame and acceptance as being the true king of his heavyweight era -- and had Tate won and then lost to Holmes it probably would have added even more ammunition to the case of Holmes as an ATG for unifying. Weaver goes from ham-and-egger fringe contender to world champ, and also several years of being considered a top-flight contender even after losing the belt.
The boxing world has already forgotten Clemente Sanchez, the former featherweight champion, and no one would have ever heard of him had it not been for that one punch...that straight right that he koed Kuniaki Shibata with.
Julian Jackson was comprehensively out boxed by Herol Graham, until a brutal single punch felled Graham. He was unconscious before he hit the ground. Herol never won the title.